Size Distribution Determination of Nanoparticles by Using Image Processing Technique
Digital image processing has increasingly been implemented in nanostructural analysis and would be an ideal tool to characterize the morphology and position of self-assembled nanoparticles. A simplified algorithm for determining the size and shape of the nanoparticles (NPs) from Transmission electron microscope (TEM) images was developed using MATLAB. TEM images of as-synthesized products were inspected using an image processing procedure. The user friendly Graphical User interface (GUI) tool was developed using MATLAB to measure the size of every particle from TEM image. The mean particle size of NPs calculated using this GUI tool by analyzing the N number of particle in the image. This algorithm allows to measure the size of nanoparticles successfully were difficult to analyse by electron microscope with lack of in-built software. The step by step process leads to optimize the process of nanoparticles synthesis and facilitate to generate the needed statistical analysis for medical and other applications.